- Dongles now authenticate FlowJo with other USB devices attached concurrently.
- Parameter short name ($P#S) keywords now utilized in more locations, such as Multi-Graph Color Maps. This change replaces many instances of the less informative parameter name ($P#N) keywords.
- Appropriate default scaling for BioRad ZE5 cytometers enabled.
- The word 'keygen' means a small program that can generate a cd key, activation number, license code, serial number, or registration number for a piece of software. Keygen is a shortcut word for Key Generator. A keygen is made available through crack groups free to download.
- FlowJo v9 will continue to work on Mojave and previous Apple operating systems that support 32-bit applications. FlowJo v9 will continue to authenticate with serial numbers and dongles. It's expected that macOS 10.15 Catalina will not support 32-bit applications and Apple computers that upgrade to Catalina—projected to be released Fall 2019.
- Jun 04, 2020 flowjo, flowjo software, flowjo download, flowjo free trial, flowjo tutorial, flowjob, flowjo portal, flowjo plugins, flowjo dongle, flowjo headquarters.
May 01, 2021 macOS Big Sur 11.3.1 (20E241) – Mac App Store 1 هفته ago Windows 10 X64 20H2 10in1 OEM en-US April-28 2021 1 هفته ago Windows 7 8.1 10 X64 PRO ULT ENT ESD en-US April 2021 2 هفته ago.
- Trouble when changes to multi-selected plots in the Layout Editor are performed.
- Manual Gates, when set as a Control, do not contain any cells.
- FlowJo Portal authentication dialog cannot be closed by clicking the ‘x’ icon in the top right / left hand corner there. Workaround: Click the “Cancel” icon in order to terminate the dialog.
- Authentication – 24-hour offline grace period (for FlowJo Site License serial number users) will not become active correctly for certain use cases.
- Saving a workspace from a template, in the same location as the workspace from which the template was created, without name modification will overwrite the original workspace. Workaround: Make sure to name workspaces uniquely.
- Double clicking on an ACS file will open FlowJo but not the workspace itself. Workaround: Load the ACS file by dragging and dropping it into an open FlowJo workspace, or by going through the File tab / Open dialog.
- FlowJo does not allow users to replace numbers from a keyword value series with text. Workaround: Use the Tab key on your keyboard to traverse to the correct field for keyword entry.
- Scrolling continuously to the end of a population name within the naming dialog can throw an error.
- Proliferation nodes applied to a group will not have the same functionality compared to group owned gates, i.e. proliferation nodes applied to synchronized groups will not adjust automatically when one node is altered, and the color/font-style of proliferation nodes will not match their group.
- Kinetics time scale is not always generated correctly, particularly in overlays
- Import/Export from Diva is unstable, and works best with BD(TM) FACSDiva version 8.5. Workaround: Use Diva version 8.5.
- Population Comparison statistics calculated in FlowJo v10.6.1 are not correct. Workaround: Use FlowJo v10.5 for calculating Population Comparisons.
Compensation
- Changes to the scale of parameters during use of the Compensation Wizard will reset changes made in that dialog. Workaround: Set scaling of parameters prior to compensation.
- Spectral compensation involving samples with height or width parameters is prone to generate improper matrices.
- Uncompensated parameters overlaid in Compensation Editor NxN preview are incorrectly labeled.
FlowJo Envoy Browser
- Clicking “No” to replace a file in FlowJo Envoy workflow will close the browser.
- Error when opening workspaces associated with a different FlowJo Envoy region, than the one currently being used. Workaround: Only access workspaces associated with your current FlowJo Envoy environment.
- Non-standard characters can bet entered as file or workspace names (including spaces and plus signs), which can cause problems downstream. Workaround: Make sure to use only standard alpha-numeric characters for data and analyses file naming.
Layout & Table Editors
- Header icons will not transfer from machine to machine when sharing workspaces.
- Batching layouts with NxN plots can cause corruption in workspaces. Workaround: Create NxN plots after batching.
- Batched text boxes cannot be edited.
- Control populations may lose their control status when adjusting the settings of an overlay graph.
Plugins
- No plugins will be included in FlowJo’s installer be default, as of FlowJo v10.6.1. Workaround: Plugins can be downloaded and installed from FlowJo Exchange.
- R based plugins won’t function when files or file paths include spaces or special characters. Workaround: Remove spaces and special characters from data files and file paths.
- Derived parameters generated from plugins can be lost after a workspace is saved. Workaround: Export FCS file(s) on which plugins are run including all derived parameters there, and re-import the exported files for downstream analysis.
Your Serial Number is created from the Hardware Address of your computer.
Installing Serial Numbers:
- Download the current version of FlowJo or copy FlowJo from the CD to your computer.
- Launch FlowJo by double clicking the application icon.
- Visit our licensing page for registration options, here: www.flowjo.com/solutions/flowjo/product-licensing
- Correspond with our licensing team to request your serial number.
- When it arrives by e-mail, launch FlowJo and enter the serial number in the dialog box.
- Serial numbers are issued for one year, and are renewable to give you the flexibility to assign your user license to a different Macintosh.
Trouble Shooting:
Vin Number Check
-Serial Numbers and dongles are two different methods of license protection. If you have a serial number, you do not need a dongle.
-FlowJo checks to make sure the computer's date and time (and all the files on the computer) are correct before it will run. You can use Disk First Aid or Disk Doctor to find and correct the files that are improperly dated.
-Capitals are important. It is best to copy and paste the serial number into the dialog box.
-Check the hardware address - if you are using an airport hub, this gives a different hardware address to FlowJo. We recommend turning off the Airport connection.
-The hardware address of the computer will show up at the bottom of the Serial Number dialog box. If the Serial Number dialog box does not come up automatically when FlowJo is launched, open it from under the FlowJo menu (in OSX) or the Apple menu (in OS9).
-Alternative methods for determining the hardware address:
OS9 - open the TCP/IP control panel and choose Get Info from the File menu.
OSX - open the Network dialog (from the System Preferences) and click on TCP/IP.
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